Course Description

This course introduces the basic biology of proteins and the new advanced science called as proteomics which aims to look into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. The course will cover in detail the two major aspects of proteomics i.e., Gel-based proteomics and Mass spectrometry-based proteomics. The gel-based module will cover different techniques like SDS-PAGE, 2-DE, 2D-DIGE etc. These techniques had a major contribution in transition from protein chemistry to proteomics. Mass spectrometry, on the other hand, is an advanced analytical technique for accurate mass measurement. In this module, we will discuss the basics of mass spectrometry, sample preparations, liquid chromatography, hybrid mass spectrometers and quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry. The course will also provide the basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics

learning outcomes

By the end of the course, candidates should be able to: The participants should gain experience in interpreting and presenting research literature, and presenting their own research. Discussions regarding both research and literature, and feedback on presentations are emphasized. The overall aim is that the participants should obtain an increased awareness of the current trends within the protein technology and proteomics field.

Learning Objectives

    By the end of this module, you will be able to:

  • • Understand the importance of proteomics as a part of the evolutionary omics technology .

  • • Design, analyze, evaluate a proteomic experiment

  • • Be familiar with liquid chromatography, mass spectrometer

  • • Understand the difference between genome and proteome in terms of biology and complexity

  • • Select the appropriate proteomics platform based on a given hypothetical biological question to be elucidated.

  • • Acquire practical and theoretical knowledge in proteomics

  • •Understanding how to identify proteins from mass spectrometry data

  • • Understand the analysis of post-translational modifications and protein-protein interactions

  • • On-hands experience with in-solution digestions, LC-ESI mass spectrometry and protein identification.



JCI Accredited

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